Clerodane diterpenes from Polyalthia longifolia var. pendula protect SK-N-MC human neuroblastoma cells from β-amyloid insult

Tung-Ho Wu; Yung-Yi Cheng; Jing-Ru Liou; Tzong-Der Way; Chao-Jung Chen; Yung-Husan Chen; Sheng-Chu Kuo; Mohamed El-Shazly; Fang-Rong Chang; Yang-Chang Wu; Chih-Chuang Liaw

doi:10.1039/C4RA01879F

Clerodane diterpenes from Polyalthia longifolia var. pendula protect SK-N-MC human neuroblastoma cells from β-amyloid insult†

Tung-Ho Wu,‡^ab Yung-Yi Cheng,‡^c Jing-Ru Liou,‡^a Tzong-Der Way,^d Chao-Jung Chen,^ef Yung-Husan Chen,^g Sheng-Chu Kuo,^c Mohamed El-Shazly,^h Fang-Rong Chang,^aij Yang-Chang Wu*^aklm and Chih-Chuang Liaw*^j

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* Corresponding authors

^a Graduate Institute of Natural products, Kaohsiung Medical University, Kaohsiung 807, Taiwan

^b Division of Cardiovascular Surgery, Veterans General Hospital, Kaohsiung 813, Taiwan

^c Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan

^d Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung 404, Taiwan

^e Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan

^f Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan

^g National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan

^h Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street 11566, Abassia, Cairo, Egypt

ⁱ Cancer Center, Kaohsiung Medical University Hospital, No. 100 Tz-You First Road, Kaohsiung 807, Taiwan

^j Department of Marine Biotechnology and Resource, National Sun Yat-sen University, 70 Lienhai Road, Kaohsiung 80424, Taiwan
E-mail: ccliaw@mail.nsysu.edu.tw
Fax: +886-7-525-5020
Tel: +886-7-525-2000 ext. 5058

^k School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan
E-mail: yachwu@mail.cmu.edu.tw
Fax: +886-4-22060248
Tel: +886-4-22057153

^l Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan

^m Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan

Abstract

Three new diterpenes, polylongifoliaic A (1), polylongifoliaons A (4) and B (5), together with nine known diterpenes, were isolated from the unripe fruits of Polyalthia longifolia var. pendula. The structures of the new isolates were determined by extensive spectroscopic analysis. The effects of all the isolated compounds on the viability of human neuroblastoma SK-N-MC cells under β-amyloid (Aβ)-induced neurotoxicity were evaluated. Polylongifoliaic A (1), polylongifoliaon B (5) and 8–10 improved the viability of human neuroblastoma cells (SK-N-MC cells) under Aβ-induced neurotoxicity. Among the active compounds, polylongifoliaic A (1) and polylongifoliaon B (5) exhibited the most potent activity toward SK-N-MC cells with IC₅₀ values of 1.64 μM and 3.75 μM, respectively. In addition, the isolated diterpenes were found to possess potent promising acetylcholinesterase inhibitory activity, which was revealed by the TLC bioautographic assay.

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Clerodane diterpenes from Polyalthia longifolia var. pendula protect SK-N-MC human neuroblastoma cells from β-amyloid insult†

Abstract

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Clerodane diterpenes from Polyalthia longifolia var. pendula protect SK-N-MC human neuroblastoma cells from β-amyloid insult

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