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Issue 29, 2014
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Lanthanide-doped nanoparticles for specific recognition of toll-like receptor (TLR) in human neutrophils

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Abstract

KYF4:Yb,Er nanoparticles were fabricated and functionalized with streptavidin for the recognition of toll-like receptors (TLRs) on human neutrophil through biotinylated lipopolysaccharide (biotin–LPS). X-Ray diffraction, Infrared spectroscopy, Transmission Electron Microscopy and Agarose Gel Electrophoresis were used to characterize the as-prepared and functionalized nanoparticles. Confocal Scanning Laser Microscopy was applied to study the TLR specific recognition using KYF4:Yb,Er and the uptake of the nanoparticles functionalized with BSA/Dextran–streptavidin in the presence of biotin–LPS by human neutrophils under normal (37 °C) and cell stressing conditions (4 °C). Confocal microscopy studies showed that the uptake of the functionalized KYF4:Yb,Er nanoparticles occurs faster and with a higher rate at 37 °C compared to the uptake at 4 °C, indicating that the uptake mechanism is an energy dependent process. Research reported in this work provides relevant guidance for the development of lanthanide doped KYF4:Yb,Er nanoparticles as specific intracellular probes, allowing control of the nanoparticle–cell interactions by tuning the surface properties. Furthermore, our results would pave the way to the use of lanthanide-doped materials for imaging cellular processes such as uptake of nanoparticles and recognition of receptors involved in immune responses.

Graphical abstract: Lanthanide-doped nanoparticles for specific recognition of toll-like receptor (TLR) in human neutrophils

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Supplementary files

Article information


Submitted
27 Dec 2013
Accepted
14 Mar 2014
First published
17 Mar 2014

RSC Adv., 2014,4, 15040-15047
Article type
Paper

Lanthanide-doped nanoparticles for specific recognition of toll-like receptor (TLR) in human neutrophils

I. C. Serrano, A. M. Adams, R. Palankar, G. Stoica, E. Palomares and M. Delcea, RSC Adv., 2014, 4, 15040
DOI: 10.1039/C3RA48018F

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