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Issue 23, 2014
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Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes

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Abstract

Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL−1, 187 μg and 51 min for diclofenac transformation (20 mg L−1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL−1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity.

Graphical abstract: Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes

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Article information


Submitted
22 Oct 2013
Accepted
08 Jan 2014
First published
09 Jan 2014

RSC Adv., 2014,4, 11689-11697
Article type
Paper

Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes

P. Sathishkumar, A. Mythili, T. Hadibarata, R. Jayakumar, M. S. Kanthimathi, T. Palvannan, M. Ponraj, M. R. Salim and A. R. Mohd Yusoff, RSC Adv., 2014, 4, 11689
DOI: 10.1039/C3RA46014B

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