Jump to main content
Jump to site search

Issue 11, 2014
Previous Article Next Article

Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization

Author affiliations

Abstract

The sphingolipid ceramide modulates stress-induced cell death and apoptosis. We have shown that ceramide generated via de novo sphingolipid biosynthesis is required to initiate apoptosis after photodynamic therapy (PDT). The objective of this study was to define the role of ceramide synthase (CERS) in PDT-induced cell death and apoptosis using fumonisin B1 (FB), a CERS inhibitor. We used the silicon phthalocyanine Pc4 for PDT, and SCC17B cells, as a clinically-relevant model of human head and neck squamous carcinoma. zVAD-fmk, a pan-caspase inhibitor, as well as FB, protected cells from death after PDT. In contrast, ABT199, an inhibitor of the anti-apoptotic protein Bcl2, enhanced cell killing after PDT. PDT-induced accumulation of ceramide in the endoplasmic reticulum and mitochondria was inhibited by FB. PDT-induced Bax translocation to the mitochondria and cytochrome c release were also inhibited by FB. These novel data suggest that PDT-induced cell death via apoptosis is CERS/ceramide-dependent.

Graphical abstract: Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization

Back to tab navigation

Supplementary files

Article information


Submitted
28 Jul 2014
Accepted
16 Sep 2014
First published
16 Sep 2014

Photochem. Photobiol. Sci., 2014,13, 1621-1627
Article type
Paper

Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization

N. B. Boppana, M. Kodiha, U. Stochaj, H. Lin, A. Haimovitz-Friedman, A. Bielawska, J. Bielawski, G. W. Divine, J. A. Boyd, M. Korbelik and D. Separovic, Photochem. Photobiol. Sci., 2014, 13, 1621
DOI: 10.1039/C4PP00292J

Search articles by author

Spotlight

Advertisements