Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases

Hongwang Wang; Dinusha N. Udukala; Thilani N. Samarakoon; Matthew T. Basel; Mausam Kalita; Gayani Abayaweera; Harshi Manawadu; Aruni Malalasekera; Colette Robinson; David Villanueva; Pamela Maynez; Leonie Bossmann; Elizabeth Riedy; Jenny Barriga; Ni Wang; Ping Li; Daniel A. Higgins; Gaohong Zhu; Deryl L. Troyer; Stefan H. Bossmann

doi:10.1039/C3PP50260K

Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases†

Hongwang Wang,*^a Dinusha N. Udukala,*^a Thilani N. Samarakoon,^a Matthew T. Basel,^b Mausam Kalita,^c Gayani Abayaweera,^a Harshi Manawadu,^a Aruni Malalasekera,^a Colette Robinson,^a David Villanueva,^a Pamela Maynez,^a Leonie Bossmann,^a Elizabeth Riedy,^a Jenny Barriga,^a Ni Wang,^a Ping Li,^a Daniel A. Higgins,^a Gaohong Zhu,^d Deryl L. Troyer^b and Stefan H. Bossmann*^a

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* Corresponding authors

^a Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA
E-mail: hongwang@ksu.edu, dinu@ksu.edu, sbossman@ksu.edu
Fax: +1-785-532-6666
Tel: +1-785-532-6817

^b Kansas State University, Department of Anatomy & Physiology, 130 Coles Hall, Manhattan, KS, USA
E-mail: troyer@vet.k-state.edu
Fax: +1-785-532-4557
Tel: +1-785-532-4509

^c University of Utah School of Medicine, 201 South Presidents Circle, Salt Lake City, UT 84112, USA
E-mail: mausam.kalita@utah.edu
Tel: +1-801-581-5339

^d The First Affiliated Hospital of Kunming Medical University, Department of Nuclear Medicine, 295 Xichang Road, Kunming, Yunnan, P.R. China
E-mail: zhugaohong@hotmail.com
Fax: +011-86-0871-65366959
Tel: +011-86-15987177568

Abstract

Numerous proteases are known to be necessary for cancer development and progression including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins. The goal of this research is to develop an Fe/Fe₃O₄ nanoparticle-based system for clinical diagnostics, which has the potential to measure the activity of cancer-associated proteases in biospecimens. Nanoparticle-based “light switches” for measuring protease activity consist of fluorescent cyanine dyes and porphyrins that are attached to Fe/Fe₃O₄ nanoparticles via consensus sequences. These consensus sequences can be cleaved in the presence of the correct protease, thus releasing a fluorescent dye from the Fe/Fe₃O₄ nanoparticle, resulting in highly sensitive (down to 1 × 10⁻¹⁶ mol l⁻¹ for 12 proteases), selective, and fast nanoplatforms (required time: 60 min).

This article is part of the themed collection: Dedicated to the memory of Prof. Nicholas J. Turro

Photochemical & Photobiological Sciences

Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases†

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Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases

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