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Issue 10, 2014
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Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

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Abstract

A stereodivergent plan is presented leading to all eight stereoisomers of oseltamivir carboxylate (OC). Key chemical manoeuvers are (1) a three-component vinylogous Mukaiyama–Mannich reaction, which sets the whole carbon skeleton and heteroatom substituents, and (2) an intramolecular, silylative Mukaiyama aldol reaction, which creates the targeted carbocycle. The viability of the plan was demonstrated by the first total synthesis of 4-epi-oseltamivir carboxylate (6), accessed in 15 steps from glyceraldehyde, o-anisidine and pyrrole siloxydiene precursors. Compound 6 inhibits influenza A virus strains H1N1 and H3N2 at the μM level, about 150 000-fold less than the OC reference, testifying that the stereodisposition of the C4 acetamido function is key for enzyme recognition. Guided by in-depth structural evaluation including NMR solution studies, molecular mechanics simulations, docking analyses and X-ray crystallography, rationalization of the biological verdict was established.

Graphical abstract: Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

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Article information


Submitted
17 Oct 2013
Accepted
21 Nov 2013
First published
22 Nov 2013

Org. Biomol. Chem., 2014,12, 1561-1569
Article type
Paper

Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

A. Sartori, L. Dell'Amico, L. Battistini, C. Curti, S. Rivara, D. Pala, P. S. Kerry, G. Pelosi, G. Casiraghi, G. Rassu and F. Zanardi, Org. Biomol. Chem., 2014, 12, 1561
DOI: 10.1039/C3OB42069H

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