Jump to main content
Jump to site search

Issue 20, 2014
Previous Article Next Article

Cationic polymer brush-modified cellulose nanocrystals for high-affinity virus binding

Author affiliations

Abstract

Surfaces capable of high-affinity binding of biomolecules are required in several biotechnological applications, such as purification, transfection, and sensing. Therein, the rod-shaped, colloidal cellulose nanocrystals (CNCs) are appealing due to their large surface area available for functionalization. In order to exploit electrostatic binding, their intrinsically anionic surfaces have to be cationized as biological supramolecules are predominantly anionic. Here we present a facile way to prepare cationic CNCs by surface-initiated atom-transfer radical polymerization of poly(N,N-dimethylaminoethyl methacrylate) and subsequent quaternization of the polymer pendant amino groups. The cationic polymer brush-modified CNCs maintained excellent dispersibility and colloidal stability in water and showed a ζ-potential of +38 mV. Dynamic light scattering and electron microscopy showed that the modified CNCs electrostatically bind cowpea chlorotic mottle virus and norovirus-like particles with high affinity. Addition of only a few weight percent of the modified CNCs in water dispersions sufficed to fully bind the virus capsids to form micrometer-sized assemblies. This enabled the concentration and extraction of the virus particles from solution by low-speed centrifugation. These results show the feasibility of the modified CNCs in virus binding and concentrating, and pave the way for their use as transduction enhancers for viral delivery applications.

Graphical abstract: Cationic polymer brush-modified cellulose nanocrystals for high-affinity virus binding

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Jun 2014, accepted on 31 Jul 2014 and first published on 05 Aug 2014


Article type: Paper
DOI: 10.1039/C4NR03584D
Nanoscale, 2014,6, 11871-11881

  •   Request permissions

    Cationic polymer brush-modified cellulose nanocrystals for high-affinity virus binding

    H. Rosilo, J. R. McKee, E. Kontturi, T. Koho, V. P. Hytönen, O. Ikkala and M. A. Kostiainen, Nanoscale, 2014, 6, 11871
    DOI: 10.1039/C4NR03584D

Search articles by author

Spotlight

Advertisements