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Issue 22, 2014
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High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

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Abstract

High precision, high yield, and high density self-assembly of nanoparticles into arrays is essential for nanophotonics. Spatial deviations as small as a few nanometers can alter the properties of near-field coupled optical nanostructures. Several studies have reported assemblies of few nanoparticle structures with controlled spacing using DNA nanostructures with variable yield. Here, we report multi-tether design strategies and attachment yields for homo- and hetero-nanoparticle arrays templated by DNA origami nanotubes. Nanoparticle attachment yield via DNA hybridization is comparable with streptavidin-biotin binding. Independent of the number of binding sites, >97% site-occupation was achieved with four tethers and 99.2% site-occupation is theoretically possible with five tethers. The interparticle distance was within 2 nm of all design specifications and the nanoparticle spatial deviations decreased with interparticle spacing. Modified geometric, binomial, and trinomial distributions indicate that site-bridging, steric hindrance, and electrostatic repulsion were not dominant barriers to self-assembly and both tethers and binding sites were statistically independent at high particle densities.

Graphical abstract: High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

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Supplementary files

Article information


Submitted
04 Jun 2014
Accepted
06 Oct 2014
First published
07 Oct 2014

Nanoscale, 2014,6, 13928-13938
Article type
Paper
Author version available

High precision and high yield fabrication of dense nanoparticle arrays onto DNA origami at statistically independent binding sites

S. Takabayashi, W. P. Klein, C. Onodera, B. Rapp, J. Flores-Estrada, E. Lindau, L. Snowball, J. T. Sam, J. E. Padilla, J. Lee, W. B. Knowlton, E. Graugnard, B. Yurke, W. Kuang and W. L. Hughes, Nanoscale, 2014, 6, 13928
DOI: 10.1039/C4NR03069A

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