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Issue 20, 2014
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Peptide modified gold nanoparticles for improved cellular uptake, nuclear transport, and intracellular retention

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Abstract

Gold nanoparticles (GNPs) are being extensively used in cancer therapeutic applications due to their ability to act both as an anticancer drug carrier in chemotherapy and as a dose enhancer in radiotherapy. The therapeutic response can be further enhanced if nanoparticles (NPs) can be effectively targeted into the nucleus. Here, we present an uptake and removal of GNPs functionalized with three peptides. The first peptide (RGD peptide) enhanced the uptake, the second peptide (NLS peptide) facilitated the nuclear delivery, while the third one (pentapeptide) covered the rest of the surface and protected it from the binding of serum proteins onto the NP surface. The pentapeptide also stabilized the conjugated GNP complex. The peptide-capped GNPs showed a five-fold increase in NP uptake followed by effective nuclear localization. The fraction of NPs exocytosed was less for peptide-capped NPs as compared to citrate-capped ones. Enhanced uptake and prolonged intracellular retention of peptide-capped GNPs could allow NPs to perform their desired applications more efficiently in cells. These studies will provide guidelines for developing NPs for therapeutic applications, which will require “controlling” of the NP accumulation rate while maintaining low toxicity.

Graphical abstract: Peptide modified gold nanoparticles for improved cellular uptake, nuclear transport, and intracellular retention

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Supplementary files

Article information


Submitted
09 May 2014
Accepted
04 Aug 2014
First published
07 Aug 2014

Nanoscale, 2014,6, 12026-12033
Article type
Paper
Author version available

Peptide modified gold nanoparticles for improved cellular uptake, nuclear transport, and intracellular retention

C. Yang, J. Uertz, D. Yohan and B. D. Chithrani, Nanoscale, 2014, 6, 12026
DOI: 10.1039/C4NR02535K

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