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Issue 2, 2015
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Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method

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Abstract

Giant unilamellar vesicles (GUVs) have a wide range of applications in biology and synthetic biology. As a result, new approaches for constructing GUVs using microfluidic techniques are emerging but there are still significant shortcomings in the control of fundamental vesicle structural parameters such as size, lamellarity, membrane composition and internal contents. We have developed a novel microfluidic platform to generate compositionally-controlled GUVs. Water-in-oil (W/O) droplets formed in a lipid-containing oil flow are transferred across an oil–water interface, facilitating the self-assembly of a phospholipid bilayer. In addition, for the first time we have studied the mechanical properties of the resultant lipid bilayers of the microfluidic GUVs. Using fluctuation analysis we were able to calculate the values for bending rigidity of giant vesicles assembled on chip and demonstrate that these correlate strongly with those of traditional low throughput strategies such as electroformation.

Graphical abstract: Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method

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Publication details

The article was received on 27 Oct 2014, accepted on 14 Nov 2014 and first published on 14 Nov 2014


Article type: Paper
DOI: 10.1039/C4LC01277A
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Citation: Lab Chip, 2015,15, 557-562
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    Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method

    K. Karamdad, R. V. Law, J. M. Seddon, N. J. Brooks and O. Ces, Lab Chip, 2015, 15, 557
    DOI: 10.1039/C4LC01277A

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