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Issue 20, 2014
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Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

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Abstract

A simple microfluidic platform was utilized to immobilize glucose oxidase (GOx) in a nonionic micellar scaffold. The immobilization of GOx was verified by using a combination of cryogenic electron microscopy (cryo-EM), scanning electron microscopy (SEM), and ultraviolet spectroscopy (UV) techniques. Chronoamperometric measurements were conducted on nanogel–GOx scaffolds under different glucose concentrations, exhibiting linear amperometric responses. Without impacting the lifetime and denaturation of GOx, the nonionic nanogel provides a favorable microenvironment for GOx in biological media. This flow-induced immobilization method in a nonionic nanogel host matrix opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process to immobilize biomolecules that are averse to ionic environments.

Graphical abstract: Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

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Supplementary files

Article information


Submitted
24 May 2014
Accepted
07 Aug 2014
First published
07 Aug 2014

Lab Chip, 2014,14, 3912-3916
Article type
Communication

Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

J. J. Cardiel, Y. Zhao, L. Tonggu, L. Wang, J. Chung and A. Q. Shen, Lab Chip, 2014, 14, 3912
DOI: 10.1039/C4LC00610K

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