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Issue 19, 2014
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Cross polarization compatible dialysis chip

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We visualize birefringence in microliter sample volumes using a microfluidic dialysis chip optimized for cross polarization microscopy. The chip is composed of two overlapping polydimethylsiloxane (PDMS) channels separated by a commercial cellulose ester membrane. Buffer exchange in the sample chamber is achieved within minutes by dialyzing under continuous reservoir flow. Using fd virus as a birefringent model system, we monitor the fd virus isotropic to liquid crystal phase transition as a function of ionic strength. We show that the reorientation of the fd virus spans a few tens of seconds, indicative of fast ion exchange across the membrane. Complete phase separation reorganization takes minutes to hours as it involves diffusive virus mass transport within the storage chamber.

Graphical abstract: Cross polarization compatible dialysis chip

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Article information

21 May 2014
17 Jul 2014
First published
17 Jul 2014

Lab Chip, 2014,14, 3700-3704
Article type
Technical Innovation
Author version available

Cross polarization compatible dialysis chip

M. Kornreich, M. Heymann, S. Fraden and R. Beck, Lab Chip, 2014, 14, 3700
DOI: 10.1039/C4LC00600C

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