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Issue 17, 2014
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Microfluidic cryofixation for correlative microscopy

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Abstract

Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.

Graphical abstract: Microfluidic cryofixation for correlative microscopy

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Supplementary files

Article information


Submitted
17 Mar 2014
Accepted
30 Jun 2014
First published
30 Jun 2014

Lab Chip, 2014,14, 3281-3284
Article type
Communication
Author version available

Microfluidic cryofixation for correlative microscopy

Y. X. Mejia, H. Feindt, D. Zhang, S. Steltenkamp and T. P. Burg, Lab Chip, 2014, 14, 3281
DOI: 10.1039/C4LC00333K

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