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Issue 16, 2014
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A microfluidic device for evaluating the dynamics of the metabolism-dependent antioxidant activity of nutrients

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Abstract

Various food components are known for their health-promoting effects. However, their biochemical effects are generally evaluated in vitro, and their actual in vivo effect can vary significantly, depending on their metabolic profiles. To evaluate the effect of the liver metabolism on the antioxidant activity, we have developed a two-compartment microfluidic system that integrates the dynamics of liver metabolism and the subsequent antioxidant activity of food components. In the first compartment of the device, human liver enzyme fractions were immobilized inside a poly(ethylene glycol) diacrylate (PEGDA) hydrogel to mimic the liver metabolism. The radical scavenging activity was evaluated by the change of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) absorbance in the second compartment. Reaction engineering and fluid mechanics principles were used to develop a simplified analytical model and a more complex finite element model, which were used to design the chip and determine the optimal flow conditions. For real-time measurements of the reaction on a chip, we developed a custom-made photospectrometer system with an LED light source. The developed microfluidic system showed a linear and dose-dependent antioxidant activity in response to increasing concentration of flavonoid. We also compared the antioxidant activity of flavonoid after various liver metabolic reactions. This microfluidic system can serve as a novel in vitro platform for predicting the antioxidant activity of various food components in a more physiologically realistic manner, as well as for studying the mechanism of action of such food components.

Graphical abstract: A microfluidic device for evaluating the dynamics of the metabolism-dependent antioxidant activity of nutrients

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Publication details

The article was received on 17 Mar 2014, accepted on 30 Apr 2014 and first published on 12 Jun 2014


Article type: Paper
DOI: 10.1039/C4LC00332B
Lab Chip, 2014,14, 2948-2957

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    A microfluidic device for evaluating the dynamics of the metabolism-dependent antioxidant activity of nutrients

    J. Lee, J. Choi, S. K. Ha, I. Choi, S. H. Lee, D. Kim, N. Choi and J. H. Sung, Lab Chip, 2014, 14, 2948
    DOI: 10.1039/C4LC00332B

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