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Issue 13, 2014
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A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

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Abstract

With the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes is gaining widespread importance. We present a novel, label-free microfluidic assay that quantifies the permeability coefficient of a broad spectrum fluoroquinolone antibiotic, norfloxacin, across lipid membranes using the UV autofluorescence of the drug. We use giant lipid vesicles as highly controlled model systems to study the diffusion through lipid membranes. Our technique directly determines the permeability coefficient without requiring the measurement of the partition coefficient of the antibiotic.

Graphical abstract: A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

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Article information


Submitted
19 Feb 2014
Accepted
17 Apr 2014
First published
14 May 2014

Lab Chip, 2014,14, 2303-2308
Article type
Paper
Author version available

A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

J. Cama, C. Chimerel, S. Pagliara, A. Javer and U. F. Keyser, Lab Chip, 2014, 14, 2303
DOI: 10.1039/C4LC00217B

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