Issue 11, 2014

Integrating microfluidic generation, handling and analysis of biomimetic giant unilamellar vesicles

Abstract

The key roles played by phospholipids in many cellular processes, has led to the development of model systems, to explore both lipid–lipid and lipid–peptide interactions. Biomimetic giant unilamellar vesicles represent close facsimiles of in vivo cellular membranes, although currently their widespread use in research is hindered by difficulties involving their integration into high-throughput techniques, for exploring membrane biology intensively in situ. This paper presents an integrated microfluidic device for the production, manipulation and high-throughput analysis of giant unilamellar vesicles. Its utility is demonstrated by exploring the lipid interaction dynamics of the pore-forming antimicrobial peptide melittin, assessed through the release of fluorescent dyes from within biomimetic vesicles, with membrane compositions similar to mammalian plasma membranes.

Graphical abstract: Integrating microfluidic generation, handling and analysis of biomimetic giant unilamellar vesicles

Supplementary files

Article information

Article type
Technical Innovation
Submitted
14 Feb 2014
Accepted
04 Apr 2014
First published
04 Apr 2014
This article is Open Access
Creative Commons BY license

Lab Chip, 2014,14, 1806-1810

Author version available

Integrating microfluidic generation, handling and analysis of biomimetic giant unilamellar vesicles

D. J. Paterson, J. Reboud, R. Wilson, M. Tassieri and J. M. Cooper, Lab Chip, 2014, 14, 1806 DOI: 10.1039/C4LC00199K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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