Jump to main content
Jump to site search

Issue 10, 2014
Previous Article Next Article

Three-dimensional, sharp-tipped electrodes concentrate applied fields to enable direct electrical release of intact biomarkers from cells

Author affiliations

Abstract

Biomarkers such as proteins and nucleic acids released from human cells, bacteria, and viruses offer a wealth of information pertinent to diagnosis and treatment ranging from cancer to infectious disease. The release of these molecules from within cells is a crucial step in biomarker analysis. Here we show that purely electric-field-driven lysis can be achieved, inline, within a microfluidic channel; that it can produce highly efficient lysis and biomarker release; and, further, that it can do so with minimal degradation of the released biomarkers. Central to this new technology is the use of three-dimensional sharp-tipped electrodes (3DSTEs) in lysis, which we prove using experiment and finite-element modeling produce the electric field concentration necessary for efficient cell wall rupture.

Graphical abstract: Three-dimensional, sharp-tipped electrodes concentrate applied fields to enable direct electrical release of intact biomarkers from cells

Back to tab navigation

Publication details

The article was received on 03 Feb 2014, accepted on 23 Mar 2014 and first published on 25 Mar 2014


Article type: Paper
DOI: 10.1039/C4LC00144C
Citation: Lab Chip, 2014,14, 1785-1790
  •   Request permissions

    Three-dimensional, sharp-tipped electrodes concentrate applied fields to enable direct electrical release of intact biomarkers from cells

    M. Poudineh, R. M. Mohamadi, A. Sage, L. Mahmoudian, E. H. Sargent and S. O. Kelley, Lab Chip, 2014, 14, 1785
    DOI: 10.1039/C4LC00144C

Search articles by author

Spotlight

Advertisements