Jump to main content
Jump to site search

Issue 2, 2014
Previous Article Next Article

Single-step design of hydrogel-based microfluidic assays for rapid diagnostics

Author affiliations

Abstract

For the first time we demonstrate a microfluidic platform for the preparation of biosensing hydrogels by in situ polymerization of polyethyleneglycol diacrylate (PEG-DA) in a single step. Capillary pressure barriers enable the precise formation of gel microstructures for fast molecule diffusion. Parallel arrangement of these finger structures allows for macroscopic and standard equipment readout methods. The analyte automatically fills the space in between the gel fingers by the hydrophilic nature of the gel. Introducing the functional structures in the chip fabrication allows for rapid assay customization by making surface treatment, gel curing mask alignment and washing steps obsolete. Simple handling and functionality are illustrated by assays for matrix metalloproteinase, an important factor in chronic wound healing. Assays for total protein concentration and cell counts are presented, demonstrating the possibilities for a wide range of fast and simple diagnostics.

Graphical abstract: Single-step design of hydrogel-based microfluidic assays for rapid diagnostics

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Aug 2013, accepted on 10 Oct 2013 and first published on 11 Oct 2013


Article type: Paper
DOI: 10.1039/C3LC50944C
Lab Chip, 2014,14, 378-383

  •   Request permissions

    Single-step design of hydrogel-based microfluidic assays for rapid diagnostics

    D. Puchberger-Enengl, C. Krutzler, F. Keplinger and M. J. Vellekoop, Lab Chip, 2014, 14, 378
    DOI: 10.1039/C3LC50944C

Search articles by author

Spotlight

Advertisements