Jump to main content
Jump to site search

Issue 3, 2014
Previous Article Next Article

Enhanced laccase stability through mediator partitioning into hydrophobic ionic liquids

Author affiliations

Abstract

Laccase-mediator systems have numerous potential uses for green oxidations, but their practical use may be limited because the reactive, oxidised mediators deactivate the enzyme. TEMPO, 4-hydroxybenzyl alcohol, phenothiazine and 2-hydroxybiphenyl caused almost complete deactivation of laccase from Trametes versicolor within 24–140 h. By contrast, 18% activity was retained after 188 h in controls without mediator, and 15% in the presence of ABTS. A biphasic reaction system was developed to protect the laccase, by partitioning the mediator into water-immiscible ionic liquids. In the presence of [C6mim][AOT], laccase retained 54, 35, 35 and 41% activity after 188 h in the presence of 4-hydroxybenzyl alcohol, phenothiazine and 2-hydroxybiphenyl and ABTS, respectively, whilst 30% activity was retained in the presence of [N1 8 8 8][Sac] and TEMPO. The protection against deactivation by the mediators correlated strongly with the distribution coefficients of the mediators between ionic liquids and water.

Graphical abstract: Enhanced laccase stability through mediator partitioning into hydrophobic ionic liquids

Back to tab navigation

Publication details

The article was received on 23 Oct 2013, accepted on 05 Dec 2013 and first published on 05 Dec 2013


Article type: Paper
DOI: 10.1039/C3GC42189A
Author version
available:
Download author version (PDF)
Green Chem., 2014,16, 1462-1469
  • Open access: Creative Commons BY license
  •   Request permissions

    Enhanced laccase stability through mediator partitioning into hydrophobic ionic liquids

    L. Rehmann, E. Ivanova, H. Q. N. Gunaratne, K. R. Seddon and G. Stephens, Green Chem., 2014, 16, 1462
    DOI: 10.1039/C3GC42189A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements