Jump to main content
Jump to site search

Issue 5, 2015
Previous Article Next Article

Efficient nitrogen-13 radiochemistry catalyzed by a highly stable immobilized biocatalyst

Author affiliations

Abstract

The continuous progress of nuclear imaging techniques demands the development and implementation of efficient, clean and sustainable synthetic routes for the preparation of novel radiotracers. This is especially challenging in the context of radiotracers labelled with short-lived positron emitters such as nitrogen-13. Biocatalysis can offer attractive alternatives to conventional chemistry, because enzymes present exquisite chemical selectivity and high turnover numbers. However, enzymes have been poorly exploited in the area of radiochemistry. Herein, we present the design and fabrication of a heterogeneous biocatalyst suitable for the reduction of [13N]NO3 to [13N]NO2. A eukaryotic nitrate reductase from Aspergillus niger was immobilized on different carriers and immobilization parameters were determined. Optimal results were obtained for agarose beads activated with a positively charged tertiary amino group (Ag-DEAE). The immobilized preparation was 12-fold more thermostable than the soluble enzyme. Biochemical characterization of the immobilized enzyme showed interesting thermally-induced hyperactivation driven by the interaction between the enzyme and the carrier. The heterogeneous biocatalyst could be re-used up to 7 reaction cycles while preserving its initial activity. Finally, to demonstrate the potential of this heterogeneous biocatalyst in the context of radiochemistry, radiosynthesis of S-[13N]nitrosoglutathione was carried out using the enzymatically produced [13N]NO2 as the labelling agent.

Graphical abstract: Efficient nitrogen-13 radiochemistry catalyzed by a highly stable immobilized biocatalyst

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Feb 2015, accepted on 22 Feb 2015 and first published on 23 Feb 2015


Article type: Paper
DOI: 10.1039/C5CY00179J
Author version
available:
Download author version (PDF)
Citation: Catal. Sci. Technol., 2015,5, 2705-2713
  • Open access: Creative Commons BY license
  •   Request permissions

    Efficient nitrogen-13 radiochemistry catalyzed by a highly stable immobilized biocatalyst

    E. S. da Silva, V. Gómez-Vallejo, J. Llop and F. López-Gallego, Catal. Sci. Technol., 2015, 5, 2705
    DOI: 10.1039/C5CY00179J

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements