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Issue 19, 2014
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A new calibration concept for a reproducible quantitative detection based on SERS measurements in a microfluidic device demonstrated on the model analyte adenine

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Abstract

This study demonstrates a new concept of calibrating surface enhanced Raman scattering (SERS) intensities without using additional substances as an internal standard and explores factors such as laser fluctuation and different Ag substrates, which affect the results of quantitative analyses based on SERS. To demonstrate the capabilities of the concept, the model analyte adenine has been chosen. A lab-on-a-chip device is applied for the measurements to guarantee consistent data recording. In order to simulate varied measuring conditions, two varying silver colloids (batch 1 and 2) are utilized as a SERS substrate and two different laser power levels (25 or 55 mW) are applied on the sample. A concentration gradient was generated which allows the use of the analyte itself for the correction of the resulting SERS spectra regarding intensity deviations caused by different ambient conditions. In doing so, a vast improvement in the quantification using SERS, especially in view of the comparability, reproducibility and repeatability, is demonstrated.

Graphical abstract: A new calibration concept for a reproducible quantitative detection based on SERS measurements in a microfluidic device demonstrated on the model analyte adenine

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Publication details

The article was received on 17 Dec 2013, accepted on 17 Mar 2014 and first published on 17 Mar 2014


Article type: Paper
DOI: 10.1039/C3CP55312D
Citation: Phys. Chem. Chem. Phys., 2014,16, 9056-9063
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    A new calibration concept for a reproducible quantitative detection based on SERS measurements in a microfluidic device demonstrated on the model analyte adenine

    E. Kämmer, K. Olschewski, T. Bocklitz, P. Rösch, K. Weber, D. Cialla and J. Popp, Phys. Chem. Chem. Phys., 2014, 16, 9056
    DOI: 10.1039/C3CP55312D

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