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Issue 25, 2015
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Collagen labelling with an azide-proline chemical reporter in live cells

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Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

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Supplementary files

Article information


Submitted
08 Oct 2014
Accepted
17 Nov 2014
First published
18 Nov 2014

This article is Open Access

Chem. Commun., 2015,51, 5250-5252
Article type
Communication
Author version available

Collagen labelling with an azide-proline chemical reporter in live cells

B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250
DOI: 10.1039/C4CC07974D

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