Jump to main content
Jump to site search

Issue 86, 2014
Previous Article Next Article

eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Author affiliations

Abstract

We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

Graphical abstract: eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

Back to tab navigation

Supplementary files

Article information


Submitted
27 Aug 2014
Accepted
09 Sep 2014
First published
09 Sep 2014

Chem. Commun., 2014,50, 13153-13156
Article type
Communication
Author version available

eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA–gold affinity interactions

A. A. I. Sina, S. Howell, L. G. Carrascosa, S. Rauf, M. J. A. Shiddiky and M. Trau, Chem. Commun., 2014, 50, 13153
DOI: 10.1039/C4CC06732K

Social activity

Search articles by author

Spotlight

Advertisements