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Issue 94, 2014
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Protein stapling via azide–alkyne ligation

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Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

Graphical abstract: Protein stapling via azide–alkyne ligation

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The article was received on 19 Aug 2014, accepted on 08 Oct 2014 and first published on 09 Oct 2014

Article type: Communication
DOI: 10.1039/C4CC06528J
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Citation: Chem. Commun., 2014,50, 14900-14903

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    Protein stapling via azide–alkyne ligation

    D. M. Abdeljabbar, F. J. Piscotta, S. Zhang and A. James Link, Chem. Commun., 2014, 50, 14900
    DOI: 10.1039/C4CC06528J

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