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Issue 94, 2014
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Protein stapling via azide–alkyne ligation

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Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

Graphical abstract: Protein stapling via azide–alkyne ligation

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Article information

19 Aug 2014
08 Oct 2014
First published
09 Oct 2014

Chem. Commun., 2014,50, 14900-14903
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Author version available

Protein stapling via azide–alkyne ligation

D. M. Abdeljabbar, F. J. Piscotta, S. Zhang and A. James Link, Chem. Commun., 2014, 50, 14900
DOI: 10.1039/C4CC06528J

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