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Issue 75, 2014
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Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

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Abstract

We describe a general and simple method to identify catalytically and structurally important nucleotides in functional RNAs. Our approach is based on statistical replacement of each nucleoside with a non-nucleosidic spacer (C3 linker, Δ), followed by separation of active library variants and readout of interference effects by analysis of enzymatic primer extension reactions.

Graphical abstract: Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

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Publication details

The article was received on 21 Jun 2014, accepted on 22 Jul 2014 and first published on 28 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC04719B
Chem. Commun., 2014,50, 10937-10940

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    Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA

    K. Wawrzyniak-Turek and C. Höbartner, Chem. Commun., 2014, 50, 10937
    DOI: 10.1039/C4CC04719B

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