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Issue 70, 2014
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Engineering β-lactamase zymogens for use in protease activity assays

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Abstract

In this study, the concept of autoinhibition, a mechanism of protein activity regulation, was applied to the design and engineering of a β-lactamase zymogen. Using this zymogen, a sensitive protease assay method was developed in which activation of the zymogen by proteases produces an amplified absorbance signal. The approach reported here can be adapted for engineering of zymogens as biological sensors and components of synthetic signaling pathways.

Graphical abstract: Engineering β-lactamase zymogens for use in protease activity assays

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Submitted
16 Jun 2014
Accepted
11 Jul 2014
First published
11 Jul 2014

Chem. Commun., 2014,50, 10155-10157
Article type
Communication
Author version available

Engineering β-lactamase zymogens for use in protease activity assays

H. Kim, H. K. Yoon and T. H. Yoo, Chem. Commun., 2014, 50, 10155
DOI: 10.1039/C4CC04549A

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