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Issue 70, 2014
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Engineering β-lactamase zymogens for use in protease activity assays

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Abstract

In this study, the concept of autoinhibition, a mechanism of protein activity regulation, was applied to the design and engineering of a β-lactamase zymogen. Using this zymogen, a sensitive protease assay method was developed in which activation of the zymogen by proteases produces an amplified absorbance signal. The approach reported here can be adapted for engineering of zymogens as biological sensors and components of synthetic signaling pathways.

Graphical abstract: Engineering β-lactamase zymogens for use in protease activity assays

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Publication details

The article was received on 16 Jun 2014, accepted on 11 Jul 2014 and first published on 11 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC04549A
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Chem. Commun., 2014,50, 10155-10157

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    Engineering β-lactamase zymogens for use in protease activity assays

    H. Kim, H. K. Yoon and T. H. Yoo, Chem. Commun., 2014, 50, 10155
    DOI: 10.1039/C4CC04549A

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