Using a mutant pyrrolysyl-tRNA synthetase-tRNAPylCUA pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
A. Tuley, Y. Lee, B. Wu, Z. U. Wang and W. R. Liu, Chem. Commun., 2014, 50, 7424 DOI: 10.1039/C4CC02000F
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