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Issue 38, 2014
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A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

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Abstract

A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible site-specific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.

Graphical abstract: A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

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Article information


Submitted
28 Feb 2014
Accepted
21 Mar 2014
First published
21 Mar 2014

Chem. Commun., 2014,50, 4891-4894
Article type
Communication
Author version available

A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

H. Zhang, Y. Song, Y. Zou, Y. Ge, Y. An, Y. Ma, Z. Zhu and C. J. Yang, Chem. Commun., 2014, 50, 4891
DOI: 10.1039/C4CC01528B

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