Issue 46, 2014
From the journal:

Chemical Communications

Synthesis and evaluation of turn-on fluorescent probes for imaging steroid sulfatase activities in cells†

Chih-Hsuan Tai,‡a   Chun-Ping Lu,‡bc   Shih-Hsiung Wu*ac  and  Lee-Chiang Lo*a  

* Corresponding authors

a Department of Chemistry, National Taiwan University, Taipei 106, Taiwan
E-mail: lclo@ntu.edu.tw
Fax: +886-2-33668670
Tel: +886-2-33661669

b Department of Food Science, Fu Jen Catholic University, New Taipei City 205, Taiwan
E-mail: 089984@mail.fju.edu.tw
Fax: +886-2-22093271
Tel: +886-2-29052063

c Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan
E-mail: shwu@gate.sinica.edu.tw
Fax: +886-2-26539142
Tel: +886-2-27855696

Abstract

We have synthesized and evaluated two self-immobilizing, turn-on fluorescent probes carrying a coumarin molecular framework for imaging intracellular human steroid sulfatase (STS) activity. The 8-fluoromethyl coumarin derivative, which gives stronger fluorescence response in the incubation study with STS preparations, was successfully applied to visualize STS activity in cells.

Graphical abstract: Synthesis and evaluation of turn-on fluorescent probes for imaging steroid sulfatase activities in cells

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Article information

DOI
https://doi.org/10.1039/C4CC01282H
Article type
Communication
Submitted
18 Feb 2014
Accepted
16 Apr 2014
First published
16 Apr 2014

Chem. Commun., 2014,50, 6116-6119

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Synthesis and evaluation of turn-on fluorescent probes for imaging steroid sulfatase activities in cells

C. Tai, C. Lu, S. Wu and L. Lo, Chem. Commun., 2014, 50, 6116 DOI: 10.1039/C4CC01282H

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