Jump to main content
Jump to site search

Issue 9, 2014
Previous Article Next Article

Precise patterning of the SEBS surface by UV lithography to evaluate the platelet function through single platelet adhesion

Author affiliations

Abstract

Platelets have exhibited capabilities beyond clotting in recent years. Most of their functions are related to the nature of platelet adhesion. Establishing a facile method to understand the platelet adhesion and assess the platelet function through the mechanism and mechanics of adhesion is highly desired. Here, we report a generally applicable UV lithography technique with a photomask, which performs selective surface functionalization on large substrate areas, for creating stable, physical adhesive sites in the range of 12 μm to 3 μm. Our study demonstrated that the patterned surface facilitated probing of single platelet adhesion in a quantitative manner, and rendered platelets sensitive to adhesive proteins even at a low protein concentration. In addition, the platelet function in the presence of antiplatelet (anticancer) agents on platelets could be accurately estimated based on single platelet adhesion (SPA). This work paves a new way to understand and assess the blood platelet function. The SPA assay methodology has the potential to enable a rapid, accurate point-of-care platform suitable for evaluation of platelet function, detection of dysfunctional platelets, and assay of drug effects on platelets in cancer patients.

Graphical abstract: Precise patterning of the SEBS surface by UV lithography to evaluate the platelet function through single platelet adhesion

Back to tab navigation

Supplementary files

Article information


Submitted
06 Mar 2014
Accepted
04 Apr 2014
First published
30 Apr 2014

Biomater. Sci., 2014,2, 1186-1194
Article type
Paper
Author version available

Precise patterning of the SEBS surface by UV lithography to evaluate the platelet function through single platelet adhesion

W. Ye, Q. Shi, S. Wong, J. Hou, X. Xu and J. Yin, Biomater. Sci., 2014, 2, 1186
DOI: 10.1039/C4BM00072B

Social activity

Search articles by author

Spotlight

Advertisements