Issue 20, 2014

Amperometric biosensor precision improvement: application to organophosphorus pesticide determination

Abstract

An approach for precision improvement in organophosphorus pesticide determination was developed, applied and characterized. The enzyme organophosphorus hydrolase was employed as a specific agent for electrode surface modification, and the pulsed amperometric detection for 66.6 ms was applied for the hydrolysis product detection. Pulsed electrochemical cleaning, i.e. oxidation at a very positive potential (+1.4 V vs. Ag, AgCl) was applied every 233 ms for 166.6 ms for elimination of the fouling effect, which degrades the determination precision. By this way a RSD of 0.41% at 40 μmol L−1p-nitrophenol determination was achieved after 10 determinations, instead of 43.93% without the pulsed cleaning application.

Graphical abstract: Amperometric biosensor precision improvement: application to organophosphorus pesticide determination

Article information

Article type
Paper
Submitted
29 Jul 2014
Accepted
18 Aug 2014
First published
18 Aug 2014

Anal. Methods, 2014,6, 8232-8238

Amperometric biosensor precision improvement: application to organophosphorus pesticide determination

M. Stoytcheva, R. Zlatev, V. Gochev, Z. Velkova and G. Montero, Anal. Methods, 2014, 6, 8232 DOI: 10.1039/C4AY01792G

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