Issue 20, 2014

Magnetic solid-phase extraction of proteins based on hydroxy functional ionic liquid-modified magnetic nanoparticles

Abstract

Hydroxy functional ionic liquids have been first designed and modified on the surface of silica-coated magnetic Fe3O4 nanoparticles (MNPs) for the investigation of the extraction performance of proteins by magnetic solid-phase extraction (MSPE) and characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). BSA was chosen as a model protein to investigate the effect of extraction parameters. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions of the extraction, and the extraction efficiency could reach up to 86.92%. The RSD of extraction efficiencies in the precision, repeatability and stability experiments were 1.17% (n = 3), 3.79% (n = 3) and 4.82% (n = 3), respectively. Compared with the other four types of ionic liquids, hydroxy functional ionic liquids-modified magnetic nanoparticles have the highest extraction rates. The desorption of proteins was up to 94.91% when the concentrations of NaCl were greater than 1.1 mol Lāˆ’1. Nearly 95% of MNPs could be recovered from each run, and extraction rates decreased significantly after five runs.

Graphical abstract: Magnetic solid-phase extraction of proteins based on hydroxy functional ionic liquid-modified magnetic nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
29 Jul 2014
Accepted
07 Aug 2014
First published
08 Aug 2014

Anal. Methods, 2014,6, 8358-8367

Author version available

Magnetic solid-phase extraction of proteins based on hydroxy functional ionic liquid-modified magnetic nanoparticles

J. Chen, Y. Wang, X. Ding, Y. Huang and K. Xu, Anal. Methods, 2014, 6, 8358 DOI: 10.1039/C4AY01786B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements