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Issue 20, 2014
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Validation of HPLC assay for the identification and quantification of anthocyanins in black currants

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Abstract

Many publications describe separation and quantification techniques for anthocyanins. However, chromatographic conditions to optimize the separation were seldom reported. We developed a sensitive and reliable HPLC method and validated the method for anthocyanin separation, identification, and quantification. Separation was performed on a Zorbax SB C18 column. The conditions including ion pair reagent, mobile phase pH, and mobile phase flow rate were optimized to improve the separation efficiency. Tandem MS was used to identify the total 6 anthocyanins in black currant. Validation of the developed method showed good linearity, precision, repeatability, and excellent recovery. The validated method was used to quantify anthocyanins from black currant samples.

Graphical abstract: Validation of HPLC assay for the identification and quantification of anthocyanins in black currants

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Publication details

The article was received on 24 Jun 2014, accepted on 02 Aug 2014 and first published on 04 Aug 2014


Article type: Paper
DOI: 10.1039/C4AY01500B
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Citation: Anal. Methods, 2014,6, 8141-8147
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    Validation of HPLC assay for the identification and quantification of anthocyanins in black currants

    X. Chen, J. Parker, C. G. Krueger, D. Shanmuganayagam and J. D. Reed, Anal. Methods, 2014, 6, 8141
    DOI: 10.1039/C4AY01500B

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