Jump to main content
Jump to site search

Issue 15, 2014
Previous Article Next Article

Dispersive liquid–liquid microextraction using ammonium O,O-diethyl dithiophosphate (DDTP) as chelating agent and graphite furnace atomic absorption spectrometry for the determination of silver in biological samples

Author affiliations

Abstract

A new method for the determination of silver in biological samples is presented in this work. The method involves application of dispersive liquid–liquid microextraction (DLLME) employing ammonium O,O-diethyl dithiophosphate (DDTP) as the chelating agent for extraction and preconcentration of silver prior to quantification using graphite furnace atomic absorption spectrometry (GFAAS). Chloroform and acetone were selected as the extracting and dispersing solvent, respectively, at optimized volumes of 80 μL and 500 μL, respectively. The concentration of DDTP and the extraction time were optimized as 0.01% (m/v) and 10 min, respectively. Pyrolysis (1100 °C) and atomization (1800 °C) temperatures were optimized using a L'vov platform treated with 400 μg of tungsten as a permanent chemical modifier. The method was proven virtually free from interference from major constituents of biological samples. A detection limit of 2 ng g−1 was obtained with relative standard deviations better than 13% and an enhancement factor of 70 was achieved. The determined concentrations for Ag in certified reference biological samples were in good agreement with the certified values at a 95% statistical confidence limit. The reported method using DLLME and GFAAS presented good analytical performance for Ag determination when compared to other methods available in the literature.

Graphical abstract: Dispersive liquid–liquid microextraction using ammonium O,O-diethyl dithiophosphate (DDTP) as chelating agent and graphite furnace atomic absorption spectrometry for the determination of silver in biological samples

Back to tab navigation

Article information


Submitted
24 May 2014
Accepted
06 Jun 2014
First published
12 Jun 2014

Anal. Methods, 2014,6, 5584-5589
Article type
Paper

Dispersive liquid–liquid microextraction using ammonium O,O-diethyl dithiophosphate (DDTP) as chelating agent and graphite furnace atomic absorption spectrometry for the determination of silver in biological samples

I. M. Dittert, L. Vitali, E. S. Chaves, T. A. Maranhão, D. L. G. Borges, V. T. de Fávere and A. J. Curtius, Anal. Methods, 2014, 6, 5584
DOI: 10.1039/C4AY01247J

Social activity

Search articles by author

Spotlight

Advertisements