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Issue 14, 2014
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Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

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Abstract

We developed an electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization. Using ZF-GDH, we could specifically detect 10 copies of genomic DNA derived from Escherichia coli O157.

Graphical abstract: Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

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Publication details

The article was received on 24 Apr 2014, accepted on 22 May 2014 and first published on 22 May 2014


Article type: Communication
DOI: 10.1039/C4AY00977K
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Citation: Anal. Methods, 2014,6, 4991-4994
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    Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

    J. Lee, A. Tatsumi, K. Abe, W. Yoshida, K. Sode and K. Ikebukuro, Anal. Methods, 2014, 6, 4991
    DOI: 10.1039/C4AY00977K

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