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Issue 14, 2014
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Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

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Abstract

We developed an electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization. Using ZF-GDH, we could specifically detect 10 copies of genomic DNA derived from Escherichia coli O157.

Graphical abstract: Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

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Article information


Submitted
24 Apr 2014
Accepted
22 May 2014
First published
22 May 2014

Anal. Methods, 2014,6, 4991-4994
Article type
Communication
Author version available

Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein

J. Lee, A. Tatsumi, K. Abe, W. Yoshida, K. Sode and K. Ikebukuro, Anal. Methods, 2014, 6, 4991
DOI: 10.1039/C4AY00977K

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