Issue 11, 2014
From the journal:

Analytical Methods

A plasmon resonance light scattering assay of glucose based on the formation of gold nanoparticles†

Wen Bi Wu,a   Lei Zhan,b   Jian Wang*a  and  Cheng Zhi Huang*ab  

* Corresponding authors

a Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
E-mail: wj123456@swu.edu.cn, chengzhi@swu.edu.cn
Fax: +86 23 68367257
Tel: +86 23 68254659

b College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China

Abstract

A simple and label-free plasmon resonance light scattering (PRLS) assay for glucose was developed based on the formation of gold nanoparticles (AuNPs) from the redox between chlorauric acid (HAuCl4) and hydrogen peroxide (H2O2) in the presence of MES. It was found that the PRLS signals characterized at 550 nm were proportional to the content of H2O2, which could be produced in the process of biocatalytic reaction of glucose oxidase (GOx), and thus a RLS method of glucose was proposed with the linear range of 5.0 × 10−6 M to 1.0 × 10−4 M and the detection limit of 2.7 × 10−6 M.

Graphical abstract: A plasmon resonance light scattering assay of glucose based on the formation of gold nanoparticles

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Article information

DOI
https://doi.org/10.1039/C4AY00206G
Article type
Paper
Submitted
23 Jan 2014
Accepted
21 Mar 2014
First published
25 Mar 2014

Anal. Methods, 2014,6, 3779-3783

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A plasmon resonance light scattering assay of glucose based on the formation of gold nanoparticles

W. B. Wu, L. Zhan, J. Wang and C. Z. Huang, Anal. Methods, 2014, 6, 3779 DOI: 10.1039/C4AY00206G

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