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Issue 2, 2014
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PCR – the polymerase chain reaction

Abstract

DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized the way that deoxyribonucleic acid (DNA) could be copied. Mullis's invention allowed researchers to make millions of copies of a selected DNA region within hours. Today, PCR can take minutes. PCR has widespread analytical applications in the food, environmental, medical and forensic fields. This Technical Brief covers the basics of PCR and some of its variations.

Graphical abstract: PCR – the polymerase chain reaction

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Article information


Submitted
19 Nov 2013
Accepted
19 Nov 2013
First published
10 Dec 2013

Anal. Methods, 2014,6, 333-336
Article type
AMC Technical Brief

PCR – the polymerase chain reaction

Analytical Methods Committee, AMCTB No 59, Anal. Methods, 2014, 6, 333
DOI: 10.1039/C3AY90101G

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