A microfluidic assay based on loop-mediated isothermal amplification was successfully developed to analyse Staphylococcus aureus in clinical cerebrospinal fluid samples. A set of primers was designed showing high specificity and good sensitivity. The reagent consumption was reduced to 1.5 μl and the total analysis time is about 60 minutes.
H. Yuan, Y. Liu, X. Jiang, S. Xu and G. Sui, Anal. Methods, 2014, 6, 2015 DOI: 10.1039/C3AY41925H
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