Issue 6, 2014

Simultaneous analysis of gemifloxacin mesylate and its main synthetic impurity by an optimized capillary zone electrophoretic method

Abstract

A simple and rapid stability indicating capillary zone electrophoretic (CZE) method was validated for the simultaneous determination of gemifloxacin mesylate (GFM) and its main synthetic impurity. The method was performed with a fused-silica capillary (50 μm i.d.) maintained at 30 °C. The background electrolyte consisted of 25 mM borate buffer (pH 10) and the separation was achieved applying 30 kV. The samples were injected hydrodynamically (6 s at 50 mbar) and detection was done at 220 nm. The procedure was validated by specificity, limit of detection (LOD), limit of quantification (LOQ), linearity, accuracy, precision and robustness. Degradations were performed in 0.1 M HCl, 0.01 M NaOH, 30% H2O2, moist heat at 60 °C and exposure to UV-A radiation to show the specificity and stability-indicating capability. Otherwise the excipients did not interfere with the quantitation of GFM. The method was linear for GFM (20.0–80.0 μg mL−1) and its synthetic impurity (3.0–20.0 μg mL−1). LOD and LOQ were 1.0 and 3.0 μg mL−1, respectively, for synthetic impurity. The results of precision and accuracy showed values that agreed with the literature and the Plackett–Burman experimental design applied confirmed the robustness. The proposed method was used for the quantitative analysis of GFM and its synthetic impurity.

Graphical abstract: Simultaneous analysis of gemifloxacin mesylate and its main synthetic impurity by an optimized capillary zone electrophoretic method

Article information

Article type
Paper
Submitted
30 Oct 2013
Accepted
31 Dec 2013
First published
08 Jan 2014

Anal. Methods, 2014,6, 1657-1665

Simultaneous analysis of gemifloxacin mesylate and its main synthetic impurity by an optimized capillary zone electrophoretic method

C. S. Paim, F. Führ, V. Todeschini, M. Steppe and E. E. Scherman Schapoval, Anal. Methods, 2014, 6, 1657 DOI: 10.1039/C3AY41922C

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