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The proposed colorimetric assay system consisting of L-Pro group-functionalized gold nanoparticles (L-Pro–AuNPs) and Cu2+ ions can discriminate the absolute configuration of His and determine the enantiomeric excess of His.

Graphical abstract: Gold nanoparticle-based colorimetric chiral discrimination of histidine: application to determining the enantiomeric excess of histidine

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