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A homochiral MOF was used as a new chiral stationary phase and had an excellent recognition ability for various racemates in HPLC. The results indicated that the optical selectivity of the natural chiral MOF column is practical in HPLC, which promotes the application of chiral MOFs for enantioseparation.

Graphical abstract: Enantioselective chromatographic resolution using a homochiral metal–organic framework in HPLC

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