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Issue 10, 2015
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Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS

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Abstract

Efficient isolation strategies not based on epithelial biomarker expression are required to enable non-biased enrichment of circulating tumor cells (CTCs). CTCs undergoing epithelial-mesenchymal transition (EMT) may be prognostically relevant, and importantly are not detected with conventional epithelial based approaches such as CellSearch®. A method for the non-biased isolation of cancer cells within a peripheral blood sample utilizing microfluidic mixing PDMS devices functionalized with anti-CD45 is reported. The introduction of micro and nanoscale roughness using a single step treatment with sulfuric acid significantly increases the binding yield of white blood cells (WBCs) to the anti-CD45 conjugated surfaces. Up to 99.99% WBC depletion is achieved with a tumor cell recovery yield of 50%. This high level of CTC enrichment is expected to facilitate the detailed characterization of CTCs using for instance, imaging flow cytometry as demonstrated here.

Graphical abstract: Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS

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Article information


Submitted
30 Sep 2014
Accepted
27 Mar 2015
First published
27 Mar 2015

Analyst, 2015,140, 3565-3572
Article type
Paper
Author version available

Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS

L. Diéguez, M. A. Winter, K. J. Pocock, K. E. Bremmell and B. Thierry, Analyst, 2015, 140, 3565
DOI: 10.1039/C4AN01768D

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