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Issue 22, 2014
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DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition

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Abstract

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

Graphical abstract: DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition

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Publication details

The article was received on 25 Jul 2014, accepted on 13 Sep 2014 and first published on 15 Sep 2014


Article type: Communication
DOI: 10.1039/C4AN01373E
Citation: Analyst, 2014,139, 5682-5685

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    DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition

    X. Jiang, S. Guo, M. Zhang, M. Zhou and B. Ye, Analyst, 2014, 139, 5682
    DOI: 10.1039/C4AN01373E

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