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Issue 17, 2014
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Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

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Abstract

An aptamer-based competitive binding assay for one-step (i.e. no requirement of pre-treatment) quantitation of target molecules of interest has been developed. This method has been successfully employed for the fast and sensitive detection of the surface antigen of the hepatitis B virus (HBsAg). The key features of our method include its low intrinsic background noise, low costs, high resolution, and high sensitivity, enabling the detection of as low as 1.25 mIU mL−1, approximately 40-fold better than that of the most widely used Abbott Architect assay for HBsAg detection, without the tedious extraction and/or washing procedures. Moreover, this assay has better recovery and accuracy than that of conventional competitive binding assay or others for HBsAg quantitation.

Graphical abstract: Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

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Supplementary files

Article information


Submitted
06 Apr 2014
Accepted
11 Jun 2014
First published
11 Jun 2014

Analyst, 2014,139, 4310-4314
Article type
Paper
Author version available

Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen

S. Suh, S. Song, H. Oh, S. Hwang and S. S. Hah, Analyst, 2014, 139, 4310
DOI: 10.1039/C4AN00619D

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