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Issue 13, 2014
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High-throughput and automatic typing via human papillomavirus identification map for cervical cancer screening and prognosis

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Abstract

A novel human papillomavirus (HPV) typing assay for cervical cancer screening and prognosis was developed by the combination of restriction fragment length polymorphism (RFLP) and microchip electrophoresis (MCE) to achieve higher levels of sensitivity and throughput. The detection limit of 2 × 102 copies, high sensitivity and typing accuracy on the account of PCR-RFLP-MCE method guarantee the successful diagnosis results of 4-fold higher infection rate over cytologic tests. From clinical samples, eleven kinds of HPV types were identified with a good compatibility degree of over 90%. The described method showed good reliability in clinical samples and provided a promising alternative for pathological studies at the molecular level.

Graphical abstract: High-throughput and automatic typing via human papillomavirus identification map for cervical cancer screening and prognosis

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Supplementary files

Article information


Submitted
16 Feb 2014
Accepted
17 Apr 2014
First published
22 Apr 2014

Analyst, 2014,139, 3330-3335
Article type
Paper

High-throughput and automatic typing via human papillomavirus identification map for cervical cancer screening and prognosis

L. Yi, X. Xu, X. Lin, H. Li, Y. Ma and J. Lin, Analyst, 2014, 139, 3330
DOI: 10.1039/C4AN00329B

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