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Issue 13, 2014
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The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells

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Abstract

We present a rapid and robust technique for the sampling of membrane-associated proteins from the surface of a single, live cell and their subsequent deposition onto a solid-supported lipid bilayer. As a proof of principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras proteins located at the inner leaflet of the plasma membrane of colon carcinoma cells and to transfer them to an S-layer supported lipid bilayer system. The technique is non-destructive, meaning that both the cell and proteins are intact after the sampling operation, offering the potential for repeated measurements of the same cell of interest. This system provides the ideal tool for the investigation of cellular heterogeneity, as well as a platform for the investigation of rare cell types such as circulating tumour cells.

Graphical abstract: The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells

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Article information


Submitted
10 Jan 2014
Accepted
20 Mar 2014
First published
20 Mar 2014

Analyst, 2014,139, 3296-3304
Article type
Paper
Author version available

The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells

A. Schrems, J. Phillips, D. Casey, D. Wylie, M. Novakova, U. B. Sleytr, D. Klug, M. A. A. Neil, B. Schuster and O. Ces, Analyst, 2014, 139, 3296
DOI: 10.1039/C4AN00059E

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