Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 13, 2014
Previous Article Next Article

Simultaneous study of subcellular exocytosis with individually addressable multiple microelectrodes

Author affiliations

Abstract

We report the application of individually addressable microelectrode arrays (MEAs) to study the heterogeneity of cell exocytosis at the subcellular level. Multiple subcellular-size electrodes are covered by a single PC12 cell for the investigation of subcellular exocytosis. PC12 cells have been seeded and cultured on top of three kinds of MEAs containing 16, 25, or 36 square microelectrodes (4 μm width in a 4 by 4 MEA, 3 μm width in a 5 by 5 MEA, 2 μm width in a 6 by 6 MEA). After collagen coating, single cells were found to cover several electrodes and these were selected for the study of subcellular exocytosis. Amperometric results show that single cell and subcellular heterogeneity in single cell exocytosis can be electrochemically detected with these MEAs. The results also show that these MEAs are suitable for detecting fast chemical events at single cells, as well as for developing multifunctional electrochemical sensors.

Graphical abstract: Simultaneous study of subcellular exocytosis with individually addressable multiple microelectrodes

Back to tab navigation

Supplementary files

Article information


Submitted
09 Jan 2014
Accepted
08 Apr 2014
First published
17 Apr 2014

Analyst, 2014,139, 3290-3295
Article type
Paper

Simultaneous study of subcellular exocytosis with individually addressable multiple microelectrodes

J. Wang and A. G. Ewing, Analyst, 2014, 139, 3290
DOI: 10.1039/C4AN00058G

Social activity

Search articles by author

Spotlight

Advertisements