Issue 7, 2014
From the journal:

Analyst

In situ molecular imaging of a hydrated biofilm in a microfluidic reactor by ToF-SIMS†

Xin Hua,‡ab   Xiao-Ying Yu,‡*a   Zhaoying Wang,‡c   Li Yang,‡c   Bingwen Liu,‡a   Zihua Zhu,‡c   Abigail E. Tucker,‡d   William B. Chrisler,‡d   Eric A. Hill,‡d   Theva Thevuthasan,‡c   Yuehe Lin,‡ef   Songqin Liu‡b  and  Matthew J. Marshall‡*d  

* Corresponding authors

a Fundamental and Computer Sciences, Pacific Northwest National Laboratory (PNNL), Richland, WA 99352, USA
E-mail: xiaoying.yu@pnnl.gov
Fax: +1-509-372-6168
Tel: +1-509-372-4524

b School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu Province, PR China

c W. R. Wiley Environmental Molecular Science Laboratory, PNNL, Richland, WA 99352, USA

d Biological Sciences Division, PNNL, Richland, WA 99352, USA
E-mail: matthew.marshall@pnnl.gov
Fax: +1-509-371-6955
Tel: +1-509-371-6964

e Physical Sciences Division, PNNL, Richland, WA 99352, USA

f School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA

Abstract

A novel microfluidic reactor for biofilm growth and in situ characterization using time-of-flight secondary ion mass spectrometry (ToF-SIMS) was constructed to enable two-dimensional chemical imaging of hydrated biofilms. We demonstrate the detection of characteristic fatty acid fragments from microfluidic reactor-grown biofilms and illustrate advantages of hydrated-state ToF-SIMS imaging.

Graphical abstract: In situ molecular imaging of a hydrated biofilm in a microfluidic reactor by ToF-SIMS

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Article information

DOI
https://doi.org/10.1039/C3AN02262E
Article type
Communication
Submitted
07 Dec 2013
Accepted
18 Jan 2014
First published
20 Jan 2014

Analyst, 2014,139, 1609-1613

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In situ molecular imaging of a hydrated biofilm in a microfluidic reactor by ToF-SIMS

X. Hua, X. Yu, Z. Wang, L. Yang, B. Liu, Z. Zhu, A. E. Tucker, W. B. Chrisler, E. A. Hill, T. Thevuthasan, Y. Lin, S. Liu and M. J. Marshall, Analyst, 2014, 139, 1609 DOI: 10.1039/C3AN02262E

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