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Issue 46, 2014
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A dual gold nanoparticle system for mesenchymal stem cell tracking

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Abstract

Stem cell-based therapies have demonstrated improved outcomes in preclinical and clinical trials for treating cardiovascular ischemic diseases. However, the contribution of stem cells to vascular repair is poorly understood. To elucidate these mechanisms, many have attempted to monitor stem cells following their delivery in vivo, but these studies have been limited by the fact that many contrast agents, including nanoparticles, are commonly passed on to non-stem cells in vivo. Specifically, cells of the reticuloendothelial system, such as macrophages, frequently endocytose free contrast agents, resulting in the monitoring of macrophages instead of the stem cell therapy. Here we demonstrate a dual gold nanoparticle system which is capable of monitoring both delivered stem cells and infiltrating macrophages using photoacoustic imaging. In vitro analysis confirmed preferential labeling of the two cell types with their respective nanoparticles and the maintenance of cell function following nanoparticle labeling. In addition, delivery of the system within a rat hind limb ischemia model demonstrated the ability to monitor stem cells and distinguish and quantify macrophage infiltration. These findings were confirmed by histology and mass spectrometry analysis. This work has important implications for cell tracking and monitoring cell-based therapies.

Graphical abstract: A dual gold nanoparticle system for mesenchymal stem cell tracking

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Publication details

The article was received on 16 Jun 2014, accepted on 08 Sep 2014 and first published on 10 Sep 2014


Article type: Paper
DOI: 10.1039/C4TB00975D
J. Mater. Chem. B, 2014,2, 8220-8230

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    A dual gold nanoparticle system for mesenchymal stem cell tracking

    L. M. Ricles, S. Y. Nam, E. A. Treviño, S. Y. Emelianov and L. J. Suggs, J. Mater. Chem. B, 2014, 2, 8220
    DOI: 10.1039/C4TB00975D

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