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Issue 30, 2014
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Functionalization of cationic poly(p-phenylene ethynylene) with dendritic polyethylene enables efficient DNAzyme delivery for imaging Pb2+ in living cells

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Abstract

We report here an effective Pb2+-dependent DNAzyme (8-17 DNAzyme) delivery system based on the water-soluble dendritic polyethylene–cationic poly(p-phenylene ethynylene) for successfully imaging Pb2+ in living cells. For utilizing the 8-17 DNAzyme and its unique ability to catalyze a phosphodiester bond cleavage reaction in the presence of Pb2+, the distinctive conjugated polymer-based polyvalent nanocarrier design manages to load and transport 8-17 DNAzyme across cell membranes, and to realize the fluorescence imaging of Pb2+ in living cells. As shown by the confocal microscopy and flow cytometry observations, the fluorescence of Cy5.5 is obviously activated under the conditions of incubation with Pb2+, compared with the absence of Pb2+. Taken together, the study demonstrates the combination of the molecular-wire effect with “dendrimer effects” on their effective DNAzyme delivery and their cellular imaging Pb2+.

Graphical abstract: Functionalization of cationic poly(p-phenylene ethynylene) with dendritic polyethylene enables efficient DNAzyme delivery for imaging Pb2+ in living cells

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Publication details

The article was received on 29 Apr 2014, accepted on 20 May 2014 and first published on 28 May 2014


Article type: Paper
DOI: 10.1039/C4TB00680A
J. Mater. Chem. B, 2014,2, 4935-4942

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    Functionalization of cationic poly(p-phenylene ethynylene) with dendritic polyethylene enables efficient DNAzyme delivery for imaging Pb2+ in living cells

    L. Zhang, H. Huang, N. Xu and Q. Yin, J. Mater. Chem. B, 2014, 2, 4935
    DOI: 10.1039/C4TB00680A

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